Lysine Propionylation Is a Prevalent Post-translational Modification in Thermus thermophilus
نویسندگان
چکیده
منابع مشابه
Lysine carboxylation: unveiling a spontaneous post-translational modification.
The carboxylation of lysine residues is a post-translational modification (PTM) that plays a critical role in the catalytic mechanisms of several important enzymes. It occurs spontaneously under certain physicochemical conditions, but is difficult to detect experimentally. Its full impact is unknown. In this work, the signature microenvironment of lysine-carboxylation sites has been characteriz...
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We report the synthesis and genetic encoding of a recently discovered post-translational modification, 2-hydroxyisobutyryl-lysine, to the genetic code of E. coli. The production of homogeneous proteins containing this amino acid will facilitate the study of modification in full-length proteins.
متن کاملLysine is synthesized through the alpha-aminoadipate pathway in Thermus thermophilus.
A 3.8-kb DNA fragment which was able to complement the mutation of a lysine auxotrophic Thermus thermophilus mutant was cloned from T. thermophilus HB27. Sequence analysis of the 3.8-kb fragment indicated the presence of three open reading frames including a truncated one. The predicted amino acid sequences of two of the three open reading frames showed 55.2% and 45.0% identity with homocitrate...
متن کاملAn extreme thermophile, Thermus thermophilus, is a polyploid bacterium.
An extremely thermophilic bacterium, Thermus thermophilus HB8, is one of the model organisms for systems biology. Its genome consists of a chromosome (1.85 Mb), a megaplasmid (0.26 Mb) designated pTT27, and a plasmid (9.3 kb) designated pTT8, and the complete sequence is available. We show here that T. thermophilus is a polyploid organism, harboring multiple genomic copies in a cell. In the cas...
متن کاملCrystal structure of a lysine biosynthesis enzyme, LysX, from Thermus thermophilus HB8.
The thermophilic bacterium Thermus thermophilus synthesizes lysine through the alpha-aminoadipate pathway, which uses alpha-aminoadipate as a biosynthetic intermediate of lysine. LysX is the essential enzyme in this pathway, and is believed to catalyze the acylation of alpha-aminoadipate. We have determined the crystal structures of LysX and its complex with ADP at 2.0A and 2.38A resolutions, r...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2014
ISSN: 1535-9476
DOI: 10.1074/mcp.m113.035659